TYR Antikörper (AA 1-433)

Produktnummer ABIN199780

Dieser Maus Monoklonal Antikörper erkennt spezifisch TYR in WB, IHC, IF, IHC (p) und ICC. Er zeigt eine Reaktivität gegenüber Human, Hund und Cat.

Kurzübersicht für TYR Antikörper (AA 1-433) (ABIN199780)

Target: TYR (Tyrosinase (TYR))

Reaktivität: Human, Hund, Cat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TYR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-TYR Antikörper

Bindungsspezifität: AA 1-433

Spezifität: Recognizes a cluster of proteins between 70-80kD, identified as tyrosinase. Occasionally a minor band at 55kD is also detected. Does not cross-react with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Species cross-reactivity: Human, canine and feline. Weak cross-reactivity with mouse.

Aufreinigung: Protein A purified

Immunogen: Recombinant tyrosinase protein. Epitope: 1-433. Cellular Localization: Cytoplasmic.

Type of Immunogen: Recombinant protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IF, IHC, IHC-P, WB (1 - 2 μg/mL)

Usage: Suitable for use in Immunocytology, Immunofluorescence, Western Blot and Immunohistology. Western Blot: 1-2 μg/mL for 2 hours at RT. Immunohistology: 2-4 μg/mL for 30 minutes at RT (formalin/paraffin). Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA,  pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Melanoma cell lines (SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37), melanocytes in skin and Melanomas (1). The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4. No preservatives or stabilizing proteins added.

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu TYR

Target: TYR (Tyrosinase (TYR))

Andere Bezeichnung: Tyrosinase

Hintergrund: Name/Gene ID: TYR

Synonyms: TYR, CMM8, LB24-AB, SHEP3, SK29-AB, Monophenol monooxygenase, Tumor rejection antigen AB, OCA1A, Tyrosinase, OCAIA

Gen-ID: 7299

UniProt: P14679